Dear Colleagues, I do have a clone for E.coli MBP- tagged RNAsin, but no information at all.

Has anyone experience with expression /purification?  I can also share the clone if someone would like to have it.

Best wishes
Sabine

Hi Sabine,

I have uploaded our protocol for MBP-RNAsin in the Files section. Just want to test if the exchange of protocols works. Would be easier to send by email of course, but who knows who else would need it.

Best regards

Hüseyin

Hi Hüseyin,

bingo!! Works nicely. Thank you again! Of course I also could have called you, but we can chat next week anyway......

Hello Dr Hüseyin Besir,

Thank you for publishing the RNasin purification protocol. I wonder if you can provide us with a gene and expression protocol.

Best regards,

Vitaly Shevchenko

Forschungszentrum Juelich, ICS-5