Service / Technology Instance

About Electron Microscopy FlexibilityHub, I2PC, Madrid, Spain

View All Electron Microscopy at Instruct

The Instruct Image Processing Center (I2PC), part of Instruct-ES, offers an extended duration service "FlexibilityHub” targeted to the exploration of the conformational landscape of flexible macromolecules from sets of cryo Electron Microscopy images of purified samples. A continuous flexibility analysis will be performed using recently developed tools (including Zernike3D, developed at I2PC, among several other methods). This service is especially suited for samples presenting large structural flexibility.

Service Availability:

Remote

Instruments Available:

Currently, I2PC mainly offer support for single particles analysis using Xmipp and Scipion softwares. Xmipp is a comprehensive suite of image processing algorithms with a strong emphasis in the analysis of single particles, although it is extending towards electron and X-ray tomography. Scipion, in turn, provides a multi-package workflow-oriented platform bridging several software suites like EMAN, Spider, Relion, Bsoft, Frealign, cryosparc and ctffind.

User Guide

The Instruct Image Processing Center (I2PC) at the National Centre of Biotechnology-CSIC is opening with the new service “Flexibility Hub”, an innovative approach for image processing of challenging samples presenting a large degree of flexibility, especially if this flexibility is continuous, making it difficult to assign images to discrete classes. As with other I2PC services, we will be using the SCIPION framework, which includes movie alignment, particle picking, classification 2D-3D, volume reconstruction and atomic structure determination; however, the added value is the use of the newly developed “Flexibility Hub” module. This allows the interoperation among several recent methods for flexibility analysis, including the I2PC developed Zernike3D approach. SCIPION provides a multi-package workflow-oriented platform bridging several software suites like Xmipp, EMAN, Spider, Relion, Bsoft, Frealign, ctffind and cryoDRGN, among others. More information about SCIPION can be found here.

 

It must be noted that this kind of project starts at the end of the standard Single Particle Analysis pipeline. That is, we assume that the particles have already been selected and reconstructed into, at least, a single class. The user must provide the particles, possible discrete classes, and their 3D alignment. If this is not the case, users should apply first to our traditional service “Electron Microscopy Image Processing”

The estimation time for each project is three weeks.

 

Instruct Centre

Instruct Image Processing Center

Instruct Image Processing Center

Universidad Autónoma de Madrid

Darwin 3

Madrid

Spain

www.i2pc.es

Contacts:

Blanca Elena Benitez Silva
Blanca Elena Benitez Silva

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Roberto Melero
Roberto Melero
Consejo Superior de Investigaciones Cientificas CSIC
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Marcos Gragera
Marcos Gragera

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