Robotein Automated Protein Production, Belgium

About Robotein Automated Protein Production, Belgium

The HT Robotein® facility at CIP and SBBC has the particularity to combine high technology laboratory instrumentation: a central liquid handling workstation, a colony picking instrument, various microplate spectrophotometers, an Octet HTX, a LabChip GXII, a protein spotter and an Infrared Imager. This specific combination of equipment offers versatile tools for the development of numerous HT applications. Here is a list of the main services that Robotein® can provide. Nonetheless, we are always open to develop new and innovative assays.

HT cloning, mutagenesis, colony picking and screening for best producers of recombinant proteins

We have developed well-established protocols for automated transformation of various bacteria and yeast strains, and for site-directed or random mutagenesis. Our platform allows automated cell cloning, high throughput colony picking, as well as cell line generation. Following automated colony picking, cultures in DeepWell plates allow selection of the best producers, together with screening for optimal culture medium. The highest expression yields can be accurately and rapidly tested on the LabChip GXII and/or the Octet HTX instruments.

HT purification of recombinant proteins

Expressed proteins can be purified in a 96-well format on the robotic workstation. Automation of this process increases throughput at each purification step, reduces human error and results in higher reproducibility with an increase in yield. The efficiency of the different purification steps can be assessed on the LabChip GXII and/or the Octet HTX equipment.

Service Availability:

Remote

Physical

Instruments Available:

The EasyPick Microlab STARlet Hamilton workstation is contained in a sterile housing with a laminar flow of air drawn through a HEPA filter. It is equipped with a high-resolution CCD camera and a light table to image colonies, thus allowing fast, reliable and economical colony picking. It can also be used to dispense culture media in Deepwell plates and can handle up to ca. 3000 clones a day.

A system (LabChip GXII, Caliper Life Sciences by Perkin Elmer) for automated electrophoretic separation of nucleic acids (RNA, DNA) and proteins provides a fast and easy way to analyse samples across a wide range of molecule and fragment sizes.

The Microlab STAR Hamilton workstation is equipped with 8 independent pipetting channels, a CO-RE 96 Probe head and a CO-RE gripping tool for plate displacement. A single position on the deck is mounted with an automated BVS Vacuum System, with pressure monitoring feature. Two Heater Shaker devices are present, together with 10 thermostated positions for 96-well PCR or microtiter plates.