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View All Protein Production at Instruct
The core facility for protein production provides comprehensive services, covering every step from DNA to purified protein. These include gene cloning into expression vectors using various techniques and heterologous expression in Escherichia coli for bacterial expression, HEK293T, Expi293, and Expi293F™ GnTI- for mammalian expression, and the baculoviral system in Sf9 or High Five™ insect cells. The expression is followed by multistep protein purification.
Cloning involves primer design and the preparation of DNA plasmids for protein expression using traditional restriction enzyme-based methods as well as restriction-free techniques. It also includes mutagenesis of existing plasmids. All constructs undergo verification by sequencing.
Literature review, cloning and vector design, protocol optimization.
We offer expression of recombinant proteins in several different expression systems.
Bacterial Expression in E. coli
We start with small-scale expression and solubilization tests to identify the optimal E. coli strain for your target protein. Based on these results, we move forward with large-scale expression cell harvesting.
Baculoviral Expression in Insect Cells
Using the baculoviral expression system in Sf9 or High Five™ insect cells, we begin with small-scale transfection and expression verification via western blot. Once confirmed, we amplify the baculovirus and scale up production for large quantities of your target protein.
Mammalian Expression Systems
We also employ mammalian expression systems using HEK293T/17SF, Expi293™, and Expi293F™ GnTI- cells. We first conduct small-scale transfection, verify expression by Western blot, and proceed to large-scale production.
We offer multistep purification for your target protein, utilizing a combination of affinity chromatography, ion exchange chromatography, and size exclusion chromatography. Our purification process ensures high purity and quality, and we deliver the protein at the desired concentration in aliquots. Along with the purified protein, we provide a detailed protocol of the whole procedure.
