We have applied some changes to ARIA and are monitoring stability, but you can continue to work as normal. We appreciate your understanding.
The Astbury Biostructure Laboratory includes the electron microscopy facility within the Faculty of Biological Sciences. We have a range of state-of-the-art equipment for transmission electron microscopy of biological specimens, from small macromolecular complexes to cells, tissues and organisms.
Our facilities are available to researchers from across the University of Leeds, and external users form academia and industry.
Our facility can operate at Biosafety Level II.
Cryo-EM grid preparation available using the Vitrobot Mark IV and Leica EM GP. Plunge-freezing systems provide precise temperature and humidity control for reliable vitrification of biological samples that can be optimised for downstream cryo-electron microscopy analysis.
The HydraBio is designed for thinning biological samples and combines focused ion beam (FIB) milling with scanning electron microscopy (SEM). It also has an in-chamber fluorescence system, allowing for correlative light and electron microscopy (CLEM) during milling.
The system is designed for rapid and highly reproducible sample vitrification. High-speed, digitally controlled blotting minimises delay between sample application and vitrification, reducing exposure to the air-water interface. The device also enables multiple modes of sample application for rapid mixing of samples and plunge-freezing to capture time-sensitive structural states.
Cryogenically cooled 300 kV transmission electron microscope, equipped with a Falcon 4i direct electron detector and a Selectris energy filter. The microscope is optimal for high-throughput data collection for single particle analysis (EPU software) and tomography (Tomography 5 and MAPS software).
We have two state-of-the-art Titan Krios microscopes equipped with direct electron detectors (DED) for high-resolution imaging. Both Krios microscopes are equipped with a Selectris energy filter and a Falcon 4i camera, ideal for tomography data collection (Tomo software) and single-particle analysis of macromolecular complexes (EPU software). We are able to support users through sample preparation workflows, all the way to sample screening and data collection.
If you already have grids prepared, you can apply for Titan Krios access for high resolution data acquisition (single particle or electron tomography). If you require assistance during project work up, for example optimisation by negative stain, making/optimising/screening cryo-EM grids, please apply for enhanced support, where we can assign the appropriate microscopes for the project stage.
Astbury Biostructure Laboratory
Astbury Centre for Structural Molecular Biology
University of Leeds
Leeds
United Kingdom
